To distinguish between direct versus indirect effects on the translatome we set out to identify mRNAs that are both enriched with TDP-43 and translationally dysregulated in motor neurons in vivo using a combination of RNA immunoprecipitations (RIP) and Translating Ribosomes Affinity Purifications (TRAP) [39, 107] in the context of TDP-43 proteinopathy. Here, TARDBP is linked to proteostasis deficiencies.