AGFG1 and infection: As shown in Fig 1D, the efficient recruitment of Sec22b-containing ERDVs to the LCV was observed at 1 h post infection in Rab6A- and Rab33B-silenced cells, similar to mock-treated cells, implying that loss of both Rab proteins does not affect the uptake of L. pneumophila or the remodeling of the LCV.