Finally, we used CIBERSORT and LM22 to jointly estimate the scores of 22 human immune cell types in PCa samples, and compared differences in the composition of immune cell types and the expression levels of five immune checkpoints (CTLA-4, PD-1, LAG-3, TIM-3 and TIGIT) between high-RS and low-RS groups. The gene discussed is CTLA4; the disease is posterior cortical atrophy.