LRRN4CL and melanoma: To confirm that the observed phenotype was not specific to B16-F0 melanoma cells, we expressed Lrrn4cl cDNA in three other mouse melanoma cell lines (HCmel12, YUMM1.7 and B16-BL6 cells [the latter of which already displays a high metastatic propensity]) and observed increased pulmonary metastatic colonisation relative to cells transfected with the empty vector alone (Fig. 2a).