To assess whether the phenotype associated with Lrrn4cl upregulation was specific to melanoma cells, we expressed the Lrrn4cl cDNA in three non-melanoma mouse cancer cell lines (MC-38 colorectal cancer cells, MB-47 bladder cancer cells and EO771 breast cancer cells) and the same effect was observed (Fig. 2b). Here, LRRN4CL is linked to colorectal cancer.