ALB and infection: Next, total activities of extracellular (proteolytic, chitinolytic, and lipolytic) enzymes (ECEs) and Pr1 family proteases collectively required for cuticle degradation during host infection (10) were quantified from the supernatants of submerged cultures from 3-day shaking incubation of a 106-conidia/ml suspension in CDB containing bovine serum albumin (BSA) as the sole nitrogen source and enzyme inducer.