In spinal cords of ALS patients, SGTA was diffusely distributed in the cytoplasm of neurons (Fig. 3a), and double immunofluorescence staining with anti-phosphorylated TDP-43 antibody revealed that neuronal cytoplasmic inclusions of ALS were not stained by SGTA antibody (Additional file 1, Fig. S4). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.