To provide clinical relevance of ATAD2 and TGF-β1 signaling, we detected mRNA expression of C/EBPβ, TGF-β1, Snail, E-cadherin, N-cadherin and Vimentin in 83 ESCC tissues and found that ATAD2 was significantly positively correlated with TGFβ1, Snail, N-cadherin and Vimentin and negatively correlated with E-cadherin (Fig. 8a-e). This evidence concerns the gene CDH2 and esophageal squamous cell carcinoma.