Using the technology of lentivirus transfection, we constructed OCI-AML3 cells expressing the circPLXNB2 shRNA and HL-60 cells overexpressing circPLXNB2. qRT-PCR was used to verify the knockdown efficiency of circPLXNB2 shRNA in OCI-AML3 cells (Fig. 2c), and the expression of circPLXNB2 was up-regulated after infection with circPLXNB2 OE into HL-60 cells (Fig. 2d). This evidence concerns the gene RUNX2 and infection.