In addition, we performed qRT-PCR (Fig. 6b, c), IHC staining (Fig. 6d, e) and Western blotting (Fig. 6f) to analyse the expression of circPLXNB2, the PLXNB2 mRNA and protein, and proteins associated with cell cycle and apoptosis in mouse xenograft tumour tissues. This evidence concerns the gene PLXNB2 and neoplasm.