K-means clustering of immune cell proportions in osteosarcoma tumors indicates the existence of three distinct immune classes (Figure 1D), namely: Cluster 1, which has the highest proportions of CD8 T cells, γδ T cells, M1 Macrophages, Mast cells and Plasma cells and the lowest proportion of M0 Macrophages; Cluster 2, in which the percentage of M0 Macrophages is the highest; and Cluster 3, which has the highest percentage of M2 Macrophages. Here, CD8A is linked to osteosarcoma.