We examined the expression of p65, one of the important subunits of NF-κB in HCC cells and found that transient transfection of SNU-449 and PLC/PRF/5 cells with SPTBN1 siRNA augmented the cellular expression level of p65 protein compared with cells transfected with control siRNA (Figure 2A-B). Here, NFKB1 is linked to hepatocellular carcinoma.