To validate the potential oncogenic role of ADSL we screened ADSL protein expression in CRC cell lines and selected the SW480 and DLD-1 cell lines, with high endogenous levels, for knock-down experiments, and the Caco-2 and HT-29 cells, with low endogenous levels, for overexpression (Figure S3A-B). The gene discussed is ADSL; the disease is colorectal carcinoma.