The cytolytic activity of TCR-TMART-1 was inhibited by increasing the percentage of tumor cells expressing PD-L1 (Figure 1D), affecting the secretion of Granzymes (Figure 1E) and pro-inflammatory cytokines in Tnull and TCR-TMART-1, including TNFα (Figure 1F), IFNγ, and IL2 (Figure 1G). This evidence concerns the gene TNF and neoplasm.