CX3CR1 and non-small cell lung carcinoma: Given that CX3CR1 and LFA-1 functionally mediated the effect of CX3CL1/ICAM-1 on NSCLC cell behavior, nude mice were inoculated intracardially with CX3CR1-KD, CX3CR1-KD+Vector, CX3CR1-KD+LFA-1 and control A549 cells to validate the effects of CX3CL1/ICAM-1 on NSCLC spinal metastases in vivo (Figure 7A).