Histological and RT-qPCR analyses showed lower CX3CL1, CX3CR1, and LFA-1 levels in spine-metastatic lesions of the CX3CR1-KD, LFA-1-KD, and CX3CR1-KD and LFA-1-KD groups than those in control groups, indicating disengagement of the CX3CL1/ICAM-1-mediated vicious cycle between circulating NSCLC cells and VBMECs (Figure S5F and S5I). Here, CX3CL1 is linked to non-small cell lung carcinoma.