As shown in Figure 4C and 4D, there was a large number of adhered cells on the stationary phase in the sicon-VBMEC group (138 ± 13 cells/well) while genetic inhibition of CX3CL1 in VBMECs obviously decreased the number of adhered cells on the fixed phase (41 ± 7 cells/well, P < 0.01), indicating the role of CX3CL1 in mediating circulating NSCLC cell adhesion to VBMECs. This evidence concerns the gene CX3CL1 and non-small cell lung carcinoma.