To determine whether CX3CL1-mediated circulating NSCLC cell adhesion to VBMECs occurred via ICAM-1 binding to LFA-1, the LFA-1 gene in A549 and H1975 cells was knocked-down using LFA-1 shRNA while the ICAM-1 gene in VBMECs was overexpressed using ICAM-1-overexpressing lentiviruses (Figure 4B, Figure S1B and S1D). The gene discussed is ICAM1; the disease is non-small cell lung carcinoma.