In the present study, we performed enrichment analyses of DEGs in AML patients with different expression levels of HOXA-AS2, which also demonstrated that HOXA-AS2 plays an essential role in AML via the PI3K-Akt, ERK, and MAPK pathways regulation, as well as by affecting cell adhesion, cell differentiation, cell migration, angiogenesis, and cell proliferation biological processes. This evidence concerns the gene AKT1 and acute myeloid leukemia.