To further investigate whether STX2 plays an important role in the biological behavior of CRC, we then analyzed STX2 mRNA expression in seven CRC cell lines and one normal colorectal mucosa cell line FHC and it was showed that STX2 expression has obviously increased in CRC cells than that in normal mucosa epithelium of FHC (Figure 2A). This evidence concerns the gene STX2 and colorectal carcinoma.