To further evaluate the functional exhaustion status of CD8+ TILs based on PD-1 and Tim-3 expression, in our series, CD8+ TIL subsets were first isolated from bladder cancer-bearing mice by a Flow Cell Sorter into the following subsets: PD-1+Tim-3-, PD-1-Tim-3+, PD-1+Tim-3+, and PD-1-Tim-3-. The gene discussed is HAVCR2; the disease is urinary bladder carcinoma.