Until now, whether PD-1 and Tim-3 are expressed by SA-granulocyte-macrophage colony-stimulating factor (SA-GM-CSF)-anchored vaccine-induced tumor antigen-specific CD8+ TILs and whether they play a role in regulating the proliferation and cytokine secretion functions of the CD8+ TILs in bladder cancer remain unknown. This evidence concerns the gene HAVCR2 and urinary bladder cancer.