To dissect the mechanism of how AR can decrease HCC cells migration and invasion capacities, we first searched some published papers that were related to cancer progression, and from which, we selected MMP1, MAOA, RPS4X, ACP5, SMAD3 and KIF4A genes, and then we conducted western blot assay to check these genes expressions when knocking down/overexpressing AR in HCC cells. The gene discussed is SMAD3; the disease is cancer.