Shiiba et al. showed that miR-325 might play a role in head and neck squamous cell carcinoma 18, Tang et al. suggested that the miR-325 may potentially function as a prognostic marker for prostate cancer 19, Wong et al. implied that miR-325 might be involved in HCC malignancy 20, Li et al. found that microRNA-325 could inhibit hepatocellular carcinoma progression by targeting high mobility group box 1 21. This evidence concerns the gene HMGB1 and hepatocellular carcinoma.