Next we conducted reverse assay to checked if shACP5 could really reverse shAR's function to HCC cells, the results showed that shACP5 could partly reverse shAR's function to HCC cells (Figure 2A-B, E), and oeACP5 could partly revers AR's function to HCC cells (Figure 2C-D, F). This evidence concerns the gene AR and hepatocellular carcinoma.