In this study, we demonstrated that decreased AR could downregulate the expression of miR-325 to promote the migration and invasion capacities of HCC by increasing ACP5 expression, thus we have discovered a new signaling pathway in the AR and HCC progression network, and at the same time, it provides the possibility of a new treatment method for inhibiting the progression of HCC. The gene discussed is ACP5; the disease is hepatocellular carcinoma.