In our study, we found that decreased AR could decrease miR-325 expression, and then increase HCC migration and invasion capacities by targeting 3'UTR of ACP5, Moreover, oemiR-325 in HCC cells could partly reverse decreased AR's function both in in vitro and in vivo experiments, which strongly proved miR-325's negative roles in regulating tumor progression. The gene discussed is ACP5; the disease is neoplasm.