To link our in vitro studies to the clinical significance, we first knocked down AR in HA22T cells and got the stable transfected cells, then we transfected the HA22T shAR cells with luciferase reporter gene [for in vivo imaging system (IVIS)] to detect tumor progression inside mouse and then transfected the HA22T shAR cells with vector control or oemiR-325 plasmids. This evidence concerns the gene AR and neoplasm.