After applying univariate Cox regression and stepwise multivariate Cox regression analyses on the 15 seed genes, six hub genes were identified to be significantly related to HCC prognosis, which were CD163, EHHADH, KIAA0101, SLC16A2, SPP1, THBS4 (Figure 3C-D). The gene discussed is PCLAF; the disease is hepatocellular carcinoma.