To explore whether Yap is involved in the tumor-promoting effects of Mct-1 in HCC, Yap expression was upregulated in Mct-1-knockdown MHCC97H cells using a Yap-overexpression plasmid, while Yap was silenced in Mct-1-overexpressing Hep3B cells using specific siRNA. The gene discussed is MCTS1; the disease is hepatocellular carcinoma.