NEFL and adrenomyeloneuropathy: With the limitation of being based on a small sample set, we separately analyzed these AMN variants and observed significantly elevated NfL levels in the premanifest CALD group when compared to non-converting AMN patients (median 14.8 [10.3–16.7] vs. 9.4 [7.8–13.8] pg/ml, p = 0.037 Fig. 1b).