Consistent with the effects of KIF18B modulation on PCa cell proliferation, we found that, compared with shCtrl-transduced cells, KIF18B overexpression significantly increased both the migration (1.00 ± 0.051 vs 1.68 ± 0.068, P < 0.01, for DU145 cells and 1.00 ± 0.16 vs 4.12 ± 0.58, P < 0.001, for PC-3 cells) and invasion (1.00 ± 0.034 vs 1.71 ± 0.13, P < 0.01, for DU145 cells and 1.00 ± 0.15 vs 2.86 ± 0.67, P < 0.001, for PC-3 cells) of DU145 and PC-3 cell lines. Here, KIF18B is linked to posterior cortical atrophy.