In the present study, we provide evidence that expression of p-PI3K (Tyr458), p-AKT (Ser473), p-mTOR (Ser2448), and p-mTOR (Ser2481) is positively regulated by KIF18 expression in DU145 and PC-3 PCa cells, thereby establishing that the role of KIF18B in promoting PCa most likely occurs via PI3K–AKT–mTOR signaling. Here, MTOR is linked to posterior cortical atrophy.