The presence of immunoglobulin M (IgM) and IgG antibodies specific for T. gondii antigens was determined during the acute phase of infection (days 4, 7, and 10 p.i.)using line blots coated with specific recombinant T. gondii tachyzoite and bradyzoite antigens (ROP1c, GRA7, GRA8, p30, and MAG1). This evidence concerns the gene CD40LG and infection.