Thus, in this study, we investigated the association between cytoplasmic and nuclear TWIST1 expression, as well as the association of CD105 expression in endothelial cells and cytoplasmic tumor cells with clinicopathological parameters and survival outcomes in patients with CRC through immunohistochemistry (IHC) staining using tissue microarray (TMA) method. The gene discussed is ENG; the disease is colorectal carcinoma.