To determine the molecular mechanism underlying increased metastatic colonization driven by NFIB, we sequenced total mRNA of tumourspheres and primary tumours derived from Nfib high‐expression models (LM1 and LM9) and Nfib low‐expression models (HR1, LM1 Nfib KO and LM9 Nfib KO). Here, NFIB is linked to neoplasm.