In results according to the presence of NAFLD, NAFLD group had significantly higher MBP and HbA1c levels relative to non-NAFLD group during school closing period, which is consistent with other existing studies that reported children affected by obesity and NAFLD had a higher cardiovascular and metabolic risk, including hypertension30,31. This evidence concerns the gene MBP and obesity due to melanocortin 4 receptor deficiency.