To compare the expression level of DUOX2 between 5 human pancreatic ductal adenocarcinoma (PDAC) cell lines (PANC-1, BxPC-3, AsPC-1, CFPAC-1, and MIA-PaCa-2) and a normal human pancreatic ductal cell line (hTERT-HPNE), RT-qPCR and Western blotting were performed. This evidence concerns the gene DUOX2 and pancreatic ductal adenocarcinoma.