This study utilized a CRISPR/Cas9 mutagenesis editing approach and found that hemizygous variants of Med12 p.Arg1784Cys caused NTD phenotypes in mice, which clearly support the results by Rocha and colleagues showing NTD in Med12 mosaic female mice (Rocha et al., 2010a). The gene discussed is MED12; the disease is neural tube defect.