Taken together, by detecting the therapeutic effect of CDK9 inhibitors on B-ALL cell lines, we confirmed that CDK9 inhibitors induced the cell apoptosis of leukemic cells by inhibiting the c-Myc-mediated glycolysis and revealed the mechanism of CDK9 inhibitors in the treatment of B-ALL (Figure 7). The gene discussed is MYC; the disease is acute lymphoblastic leukemia.