As shown in Figure 2 and Table 2, the Galβ1-3/4GlcNAc binder LEL and MAL-I, Galα1-3GalNAc binder BPL, T antigen and sialyl-T antigen binder Jacalin, Siaα2-3/6Gal binder MAL-II/SNA, GlcNAc and Galβ1-4GlcNAc binder DSA, and GSL-II showed increased NFIs in ESCC salivary glycoproteins against HV cases. This evidence concerns the gene TBXT and esophageal squamous cell carcinoma.