Initially, NOS3 expression in different cell lines was checked and the results showed that cell lines from STAD and COAD were the top two cell lines expressing the highest levels of NOS3 mRNA, and cell lines from nerve system tissues (e.g., GBM and neuroblastoma) and bone tissues (e.g., chondrosarcoma and osteosarcoma) expressed relatively lower NOS3 mRNA (Figure 2A). The gene discussed is NOS3; the disease is osteosarcoma.