Several canonical signaling pathways generally acknowledged to promote pathological behavior of malignant tumors were involved, such as “KEGG_ABC_TRANSPORTERS,” “KEGG_CALCIUM_SIGNALING_PATHWAY,” “KEGG_ECM_ RECEPTOR_INTERACTION,” “KEGG_CYTOKINE_ CYTOKINE_RECEPTOR_INTERACTION,” “KEGG_ CHEMOKINE_SIGNALING_PATHWAY” and “KEGG_MAPK_ SIGNALING_PATHWAY.” These results indicated that NOS3 might participate in multiple canonical cancer-related signaling pathways to facilitate STAD. The gene discussed is NOS3; the disease is cancer.