To analyze the possible role of PGK1 participate in the sensitivity of breast cancer cells to paclitaxel treatment, we used shPGK1-1 and shPGK1-2 lentiviruses to create stable PGK1-downregulated cell lines derived from the MDA-MB-231 (MDA-MB-231-shPGK1-1 and MDA-MB-231-shPGK1-2) and MDA-MB-468 (MDA-MB-468-shPGK1-1 and MDA-MB-468-shPGK1-2) cell lines. This evidence concerns the gene PGK1 and breast cancer.