Additionally, we performed xenograft experiments in which nude mice were injected with ISI control cells (EV, on the left flank) and cells overexpressing MEK1 WT, R96E, R113E, or R189E mutant (on the right flank), and the mice were monitored for tumor growth over 30 days (Figure 5E, left). The gene discussed is MAP2K1; the disease is neoplasm.