AKT1 and neoplasm: In another investigation, Ndreshkjana et al. (2019) summarized the anticancer effects of thymoquinone in combination with 5‐fluorouracil (5‐FU) against colorectal cancer cell lines via various mechanisms such as (a) deregulation of gene expression, (b) elimination of CD133 + CSC population, (c) downregulation of PI3K/AKT and WNT/ß‐catenin pathways, (d) eradication of propagated 3D tumor cell spheres at subtoxic doses, (e) inhibition of cell adhesion, and (f) reduction of transcriptional activity of ß‐catenin, respectively (Ndreshkjana et al., 2019).