We also examined these miRNA in ACPA+ “at-risk individuals” and demonstrated that six miRNAs (miR-126-3p, let-7d-5p, miR-431-3p, miR-221-3p, miR-24-3p, and miR-130a-3p) of the nine miRNAs identified in the RA cohort, were also significantly elevated in ACPA+ “at-risk individuals,” suggesting that these are present in the circulation prior to disease onset. Here, PRTN3 is linked to rheumatoid arthritis.