IFNA1 and viral infectious disease: Upon the viral infection of MRC-5 and HFF cells, we observed that the expression of ISGs in cells infected with the mutant virus was higher than that in cells infected with the WT virus, and the viral load in cells infected with mutant HCMV was obviously lower than that in cells infected with WT virus, suggesting that hcmv-miR-US33as-5p knockout significantly impaired viral resistance to IFN treatment.