Fig. S1), which collectively indicated significantly lower miR-362 levels relative to the NC levels (Fig. 4B), indicating successful sponge-induced miR-362 suppression in the tumors as in the cell lines, which were used to generate the tumors, described above (Fig. 1E). The protein lysates of the tumors were subjected to western blot (Fig. 4C) and immunohistochemical analyses (Fig. 4D). The results consistently showed down-regulated SMAD4 and SNAI1 and up-regulated E-cadherin levels in miR-362 knockdown tumor samples, consistent with the in vitro results. This evidence concerns the gene SMAD4 and neoplasm.