We found that ITIH1 was negatively correlated with TMB of CHOL, head and neck squamous cell carcinoma (HNSC), LUAD, PAAD, rectum adenocarcinoma (READ), STAD, and Thymoma (THYM), but positively correlated with that of Brain lower grade glioma (LGG) (Supplementary Figure 11A). This evidence concerns the gene ITIH1 and rectum adenocarcinoma.