Although all these aberrant expression levels of DPP10-AS1, ADCY1 and miR-127-3p have demonstrated in other cancers, their roles in colon cancer, especially CCSC functions still remain to be elucidated, which implored us to investigate their roles in sphere formation and colony formation abilities as well as migration and invasion of colon cancer HT-29 and SW480 stem cells. This evidence concerns the gene ADCY1 and malignant colon neoplasm.