The levels of IL-4 (87.54 ± 1.45 and 102.0 ± 4.65 pg/ml respectively), IL-5 (83.43 ± 4.23 and 82.09 ± 4.76 pg/ml respectively), and IL-13 (163.65 ± 2.34 and 139.43 ± 6.98 pg/ml respectively) were increased in the asthma and rhinitis animals on day 31 compared with those seen in the healthy animals (IL-4: 44.03 ± 3.37, IL-5: 41.23 ± 3.43, IL-13: 6087 ± 1.99 pg/ml) and a reverse trend was found in IFN-γ (asthma 25.11 ± 4.32 and rhinitis 23.64 ± 4.67 groups compared with healthy group: 51.98 ± 6.11 pg/ml) (P < 0.05). This evidence concerns the gene IL4 and asthma.