We performed spatial RNA-seq analysis on genes implicated in CHD and found that the expression of crucial transcription factors like GATA5 was enriched in the valve region, IRX4 expression was restricted to the ventricular tissue, and TBX5 was enriched in the left ventricle (Fig. 4a–b and Fig. 3d). The gene discussed is IRX4; the disease is coronary artery disorder.