To figure out which proteins play an important role in decay of PARP1 mRNA, siRNAs of RNA methylases including methyltransferase like 3 (METTL3), METTL14 or Wilms tumor 1-associating protein (WTAP) and RNA demethylases alkB homolog 5 (ALKBH5) or fat mass and obesity associated (FTO) were designed and transfected into UOK109 individually. This evidence concerns the gene METTL3 and obesity due to melanocortin 4 receptor deficiency.