Herein, we found that NONO-TFE3-fusion protein negatively regulated TRAF3IP2-AS1 and upregulation of TRAF3IP2-AS1 could inhibit cell proliferation, migration and invasion in NONO-TFE3 tRCC. Here, TFE3 is linked to renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.