To further explore the biological function of m6A modification of PARP1 mRNA in NONO-TFE3 tRCC, CCK-8, colony formation, tumor sphere formation, EdU, flow cytometry and Transwell assays were performed. This evidence concerns the gene PARP1 and renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.