Herein, we found that NONO-TFE3-fusion protein negatively regulated TRAF3IP2-AS1 and upregulation of TRAF3IP2-AS1 could inhibit cell proliferation, migration and invasion in NONO-TFE3 tRCC. The gene discussed is TRAF3IP2; the disease is renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.