Based on the target gene prediction and enrichment analyses, expression of representative predicted target mRNAs of some of the validated microRNAs were detected via RT-qPCR and these mRNAs are involved in all the treatments, including Elovl2, Lamp2, Atp6v0a1 and Wdr18 in CR, Wdr18 in EX and Atp6v0a1 and Wdr18 in HF (Fig. 7). This evidence concerns the gene ELOVL2 and hydrops fetalis.