To assess the reliability of data of RNA-seq, five genes (FOSL2, HBB, OSM, PFKFB3, PDK4) were selected in up-regulated genes and five genes (RHOB, PTGER4, CCL3L1, DENND2D, ACCS) were selected in down-regulated genes for detection in 18 CAD patients and 18 controls by RT-qPCR according to expression value and significance. The gene discussed is PFKFB3; the disease is coronary artery disorder.