In conclusion, we not only observed the degree of expression of HSDL2 and its association with clinical variables and prognosis, but also unravelled the contribution of HSDL2 with the support of cell‐based assays and the evaluation of key proteins that are involved in the regulation of signalling networks linked to proliferation as well as migration and invasion of cervical cancer cells. This evidence concerns the gene HSDL2 and cervical cancer.