Consistent with results from our analysis of the TCGA database, the subsequent expression analysis from Gene Expression Profiling and Interactive Analyses (GEPIA) database revealed that MYL1 and ACTN2 were decreasingly expressed in HNSCC patients and COL1A1, COL1A2 and COL3A1 were upregulated (Fig. 4g). The gene discussed is ACTN2; the disease is head and neck squamous cell carcinoma.