Then as shown in Fig. 4e, the expression calculation based on TCGA database showed that MYL1, ACTN2, MYH8 and MYH6 were significantly downregulated in HNSCC samples, and COL1A1, COL1A2 and COL3A1 were over-expressed. This evidence concerns the gene MYL1 and head and neck squamous cell carcinoma.