In our hands, M1 polarization was induced in HCC tumors after 4Mu treatment, the percentage of CD3+CD8+ tumor infiltrating T cells was increased, and by the reduction of CD47 expression on CSCs they might result more “visible” to the immune system, open the possibility to combine 4Mu with other immunotherapies like checkpoints inhibitors40. The gene discussed is CD8A; the disease is neoplasm.