TLR4 and cancer: We observed that after 24 h and 48 h of pre-conditioning, 4Mu-treated pMφ derived-CM reduced the expression levels of cancer stemness markers (TLR4 and CD47)30,31, and the totipotency factor Sox232 in cells (*p < 0.05, **p < 0,01 and ****p < 0,001; Kruskal–Wallis test) (Fig. 3b).