CD19 and neoplasm: The components of the cytotoxicity assay were added in three ways: a) CAR-CD19 T cells, bridging protein and target cells were added together at same time, b) CAR-CD19 T cells were added to a premixture of bridging protein and target tumor cells, which had been pre-incubated at 37°C for 10 minutes, or c) the CAR-CD19 T cells were premixed with bridging protein and pre-incubated at 37°C for 10 minutes, and then added to target tumor cells.