To study the role of CXCL14 in HNSCC and whether this role was associated with effects of NK cells in vivo, we established a tumour‐bearing mouse model using SCC7 cells and then injected CXCL14 overexpression (oe‐mCXCL14) or knockdown (sh‐mCXCL14) lentivirus or corresponding control vectors into the mice. The gene discussed is CXCL14; the disease is head and neck squamous cell carcinoma.