EZH2 and cardiac hypertrophy: After pull-down using anti-EZH2 or normal IgG antibodies, we firstly validated the RIP procedure with known EZH2-binding lncRNAs, including cardiac hypertrophy associated epigenetics regulator (Chaer), HOX transcript antisense RNA (Hotair), myocardial infarction-associated transcript 1 (Mirt1), and H19 (28, 35–37) using real-time PCR.