Hendriks et al. (2016) assessed BSEP inhibition in two long-term 3D spheroid models of HepaRG and hpHep with repeated drug exposure and bile acids co-exposure and was able to detect the cholestatic effect of several compounds, including TGZ, in both cell types. Independently of the studied mechanisms, the co-culture of hpHep (Kostadinova et al., 2013; Proctor et al., 2017; Li et al., 2020) or hepatoma cell lines (Granitzny et al., 2017) with NPCs displayed a higher sensitivity to TGZ toxicity (Kostadinova et al., 2013). This evidence concerns the gene TGM7 and hepatocellular carcinoma.